AmoyDx® MSI PCR Kit
PCR
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The AmoyDx® MSI PCR Kit is a qualitative real-time PCR assay for detecting microsatellite instability (MSI) in formalin-fixed, paraffin embedded (FFPE) colorectal cancer tissue samples. Intended for research use only (RUO), the kit requires operation by trained professionals in a controlled laboratory setting. It is compatible with the LightCycler480 II and SLAN-96S platforms for assay performance and data acquisition. Results shall be analyzed using ARAS, a dedicated software tool optimized for AmoyDx® MSI PCR Kit outputs.
The AmoyDx® MSI PCR Kit employs advanced melting curve analysis integrated with real-time PCR, leveraging specific primers and multi channel fluorescent probes (FAM, VIC, CY5) to assess instability at six key microsatellite loci (ACVR2A, PRR5-ARHGAP8, EIF4E3, IFT140, RBM14-RBM4, UBAC2). This enables precise evaluation of microsatellite instability (MSI) status in formalin-fixed, paraffin-embedded (FFPE) colorectal cancer tissue samples. Based on the number of unstable loci detected, the kit classifies samples into two categories: microsatellite instability-high (MSI-H) or non-high microsatellite instability (MSI-L/MSS). Furthermore, the kit's PCR amplification system incorporates uracil-N-glycosylase (UNG) enzyme, which selectively hydrolyzes uracil-glycosidic bonds in dU-containing amplicons, thereby minimizing false-positive results due to carryover contamination from prior PCR products.
High Concordance Rate with Fragment Analysis MSI
No need for paired normal tissue samples as control
Streamlined workflow with
10 minutes of hands-on time
100% consistency with
gold-standard testing
Fast results within 2 hours
Specifications
Tests/kit
24 (Pre-loaded)
Instrument
LighCycler480 II, SLAN-96S
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