AmoyDx® Pan Lung Cancer PCR Panel
Detection of 167 hotspot variants in EGFR/ ALK/ ROS1/ KRAS/ BRAF/ HER2/ RET/ MET/ NTRK1/ NTRK2/ NTRK3 genes.
Patients with NSCLC often harbor driver mutations in multiple oncogenes, including EGFR, ALK, ROS1, BRAF, HER2, RET, MET, NTRK, etc. The presence of gene alterations can impact the selection of and the response to targeted therapies. Testing of lung cancer for multi-gene mutations is important for identification of potentially efficacious targeted therapies. Therefore, identifying mutations in oncogenes and tailoring therapy accordingly are widely accepted in clinical cancer management. Faster detection of driver mutations enables quicker initiation of proper therapy.
AmoyDx® Pan Lung Cancer Panel is qualitative detection of 167 hotspot alterations in EGFR, ALK, ROS1, KRAS, BRAF, HER2, RET, MET, NTRK1, NTRK2 and NTRK3 genes in NSCLC at a time from a single tissue specimen.
The assay contains DNA-based mutation detection and mRNA-based fusion detection.
The RNA gene fusion detection includes two processes: 1) Reverse Transcription: extracted RNA from FFPE or fresh tumor tissue is employed in this step, reverse transcription of target RNA enables complementary DNA (cDNA) synthesis with the action of reverse transcriptase and specific primers. 2) PCR Amplification: the specific primers are designed for amplification of cDNA, and ALK, ROS1, RET, MET, NTRK1, NTRK2 and NTRK3 variant amplicon is detected by fluorescent probes.
The DNA gene mutation detection system uses ADx-ARMS technology, which comprises specific primers and fluorescent probes to detect gene mutations. During the amplification, the target mutant DNA is matched with the bases at 3’ end of the primer, and amplified efficiently, then the mutant amplicon is detected by fluorescent-labeled probes. While the wild-type DNA cannot be matched with specific primers, there is no amplification occurs.
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|Product Name||Size (test/kit)||Storage||Cat. No.|
|AmoyDx® Pan Lung Cancer Panel (Pre-loaded)||8T||-20℃||8.0131202W008J