PIK3CA Five Mutations Detection Kit
PCR
Real-time PCR Assays
1%-2%
1%-2%
Sensitivity
100%
100%
Specificity
Product Description

PIK3CA Five Mutations Detection Kit

The AmoyDx PIK3CA Five Mutations Detection Kit is a sensitive and selective assay for the detection of five mutations in the phosphoinositide-3-kinase catalytic subunit alpha (PIK3CA) gene.

It has been found that PIK3CA gene mutations exist in several types of cancers, including breast cancer and non-small-cell lung cancer. Mutation of PIK3CA may lead to persistent activation of the downstream signaling pathway associated with the development and spread of cancer.

Technological Principles

The PIK3CA five mutation kit is a real-time PCR assay. It employs mutation-specific primers and a PIK3CA-targeted proprietary fluorescent probe to detect low-copy number (1%) PIK3CA-mutant DNA in cancer tissue.

The kit enables detection of the following mutations against a background of wild-type genomic DNA.

• H1047R3140A>G

• H1047L3140A>T

• E542K (1624G>A)

• E545D (1635G>T)

• E545K (1633G>A)

Testing Procedure
Compatible Sample Types:FFPE Samples
Compatible Real-time PCR Instrument
ABI 7500
Bio-Rad CFX96
LightCycler 480 II
Stratagene Mx3000P™
Other Info.

To order, please contact sales@amoydx.com.

AmoyDx PIK3CA Five Mutations Detection Kit provides reagents to perform assays for 24 tests: 

 Product Name

 Size (test/kit)

 Storage

 Cat. No.

  PIK3CA Five Mutations Detection Kit (Bulk)

 24T

 -20℃

8.01.21601X024E

 

Publications
1. Wang, Qiang, et al. "PIK3CA mutations confer resistance to first-line chemotherapy in colorectal cancer." Cell death & disease 9.7 (2018): 1-11.
2. Trachu, N., et al. "Molecular alterations and clinical prognostic factors for cholangiocarcinoma in Thai population." OncoTargets and therapy 10 (2017): 4955.

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