PIK3CA Five Mutations Detection Kit
PCR
Real-time PCR Assays
1%-2%
1%-2%
Sensitivity
100%
100%
Specificity
Product Description

PIK3CA Five Mutations Detection Kit

The phosphoinositide-3 -kinase catalytic alpha (PIK3CA) gene produces the p110 alpha (pl 10a) protein, which is one subunit of an enzyme called phosphatidylinositol 3-kinase (PI3K). PI3K plays a key role ofPI3K/Akt signaling pathway in numerous cellular processes critical for cancer progression, including metabolism, growth, survival, and motility. Somatic mutations in the PIK3CA gene are found in many types of cancer, including approximately 40% of patients with hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-negative breast cancer. PIK3CA mutations in which lead to Activation of the PI3K pathway in breast cancer have been typically associated with resistance to endocrine therapy and poor prognosis. The clinical studies demonstrate that PI3K inhibitors has shown significantly high response rate in patients with PIK3CA-mutated breast cancer.

Intended Use

The AmoyDx® PIK3CA Five Mutations Detection Kit is a real-time PCR assay for qualitative detection of five mutations in the PIK3CA gene in human genomic DNA extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue.

The kit is for research use only, and intended to be used by trained professionals in a laboratory environment.

Testing Procedure
Compatible Sample Types:FFPE Samples
Compatible Real-time PCR Instrument
ABI 7500
LightCycler480
SLAN-96S
Rotor-Gene 6000
Rotor-Gene Q
Bio-Rad CFX96
Stratagene Mx3000P™
Other Info.

To order, please contact sales@amoydx.com.

 Product Name

 Size (test/kit)

 Storage

  PIK3CA Five Mutations Detection Kit

 24T/12T

 -20℃

 

Publications
1. Wang, Qiang, et al. "PIK3CA mutations confer resistance to first-line chemotherapy in colorectal cancer." Cell death & disease 9.7 (2018): 1-11.
2. Trachu, N., et al. "Molecular alterations and clinical prognostic factors for cholangiocarcinoma in Thai population." OncoTargets and therapy 10 (2017): 4955.

Certificate
BACK
Inquiry
Inquiry
* Required information
Manage your account info
Change your password