KRAS/NRAS/BRAF Mutations Detection Kit
PCR
Real-time PCR Assays
1%
1%
Sensitivity
100%
100%
Accuracy
Features
Selectable
- Pre-loaded and bulk formats are available.
1
- Pre-loaded and bulk formats are available.
Universal
- Compatible with tissue samples.
2
- Compatible with tissue samples.
Precise
- Top sensitivity of 1% mutant allele.
3
- Top sensitivity of 1% mutant allele.
Easy
- One-step procedure, results in 90 mins.
4
- One-step procedure, results in 90 mins.
Reliable
- Internal and external controls included.
5
- Internal and external controls included.
Product Description

KRAS/NRAS/BRAF Mutations Detection Kit

Detection of 17 KRAS mutations (exons 2, 3 and 4), 13 NRAS mutations (exons 2, 3 and 4) and six BRAF V600 mutations (exon 15)

KRAS, NRAS and BRAF genes are the key molecules in the downstream signaling pathway of epidermal growth factor receptor (EGFR). These pathways control cell proliferation,differentiation and apoptosis.Frequency of KRAS, NRAS and BRAF mutations in colorectal cancer are 36~40%, 1~6% and 8~15% respectively. Most frequent mutations occur in exons 2, 3 and 4 of RAS gene, and in codon 600 of BRAF gene.

KRAS mutations and NRAS mutations predict a lack of response to cetuximab (Erbitux®) and panitumumab (Vectibix®) therapy in colorectal cancer (CRC).[1]A number oflarge studies[2][3] have shown that cetuximab has significant efficacy in mCRCpatients with RAS wild-type tumors.And mutations in BRAF gene are a strong prognostic marker. Clinical data has shown that colorectal cancer patients with mutant BRAF gene are less likely to response to EGFR-TKIs therapy. NCCN Clinical Practice Guideline for colon cancer clearly indicates that all patients with metastatic colorectal cancer should be determined of tumor RAS (KRAS/NRAS) and BRAF genes status.

The AmoyDx®KRAS/NRAS/BRAFMutationsDetection Kit is a real-time PCR test intended for qualitative detection of 17 KRASmutations(exons 2, 3 and 4),13 NRASmutations(exons 2, 3 and 4)and six BRAF V600mutations(exon 15).

Technological Principles

The kit uses novel, patented primers and probes to detect mutations in a real-time PCR assay.

The mutant DNA is amplified accurately by the specific primers, and detected by the novel probes.

Highly Specific primers and probes, and a highly validated procedure based on Taq DNA polymerase contribute to outstanding assay sensitivity and selectivity.

Testing Procedure
Compatible Sample Types:FFPE Samples
Compatible Real-time PCR Instrument
ABI 7500
LightCycler 480 II
SLAN-96S
Stratagene Mx3000P™
Other Info.

To order, please contact sales@amoydx.com.

AmoyDx KRAS/NRAS/BRAF Mutations Detection Kits provides reagents to perform assays for 6 tests:

 Product Name

Type

Size (test/kit)

 Storage

 Ref. No.

KRAS/NRAS/BRAF Mutations Detection Kit

Pre-loaded

6

-20℃

8.01.25403W006A
8.01.25403W006D


Publications
1. Guo, Tian‐An, et al. "Clinicopathologic features and prognostic value of KRAS, NRAS and BRAF mutations and DNA mismatch repair status: A single‐center retrospective study of 1,834 Chinese patients with Stage I–IV colorectal cancer." International journal of cancer 145.6 (2019): 1625-1634.
2. Huang, Fengbo, et al. "Thyroid-Like Low-Grade Nasopharyngeal Papillary Adenocarcinoma: A Clinicopathologic Study of Five Cases and a Literature Review." American journal of clinical pathology 152.5 (2019): 582-589.

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