JAK2 Mutation Detection Kit
Real-time PCR Assays
Product Description

JAK2 Mutation Detection Kit

The JAK family of non-receptor tyrosine kinases, includes JAK1, JAK2, JAK3, and TYK2. Growth factors and cytokines can regulate gene transcription through JAK-dependent activation of signal transducer and activator of transcription (STAT). The JAK-STAT signal transduction pathway regulates cell proliferation, differentiation, apoptosis and immune regulation. Activation of JAK2 by mutation of the amino acid at position 617 (V617F ) is associated with myeloproliferative disorders, including polycythemia vera (mutations found in 90% of cases), essential thrombocythemia (50%) and idiopathic myelofibrosis (50%). JAK2 mutation is the main diagnostic indicator of MPD. The ADx JAK2 V617F Mutation Detection Kit is a highly selective and sensitive assay for detecting the V617F mutation in the  JAK2 oncogene. AmoyDx’s patented technology allows detection of 1% mutant DNA in a background of 99% normal DNA, while ensuring that false negatives are minimized.  The procedure is easilyadapted for use in high-throughput sample processing.

Breaking JAK2 News: The JAK2 V617F mutation was found in three of 24 lung cancer samples using deep exome sequenicing. If this discovery is validated it will represent a major new driver mutation in lung cancer, with the added benefit that the mutant kinase is inhibited by well-studied drugs such as gefitinib.

Lipson, D., et al. Nature Medicine, 8:3, p382. 13 Feb 2012.

Technological Principles:

The JAK2 mutation kit is a real-time PCR assay. It employs mutation-specific primers and aJAK2 V617F -targeted proprietary fluorescent probe to detect low-copy number (1%) JAK2V617F-mutant DNA in cancer tissue.

Testing Procedure
Compatible Sample Types:Whole Blood samples
Compatible Real-time PCR Instrument
ABI 7500
ABI StepOnePlus
Bio-Rad CFX96
LightCycler 480 II
Stratagene Mx3000P™
Other Info.

To order, please contact sales@amoydx.com.

AmoyDx JAK2 Mutation Detection Kit provides reagents to perform assays for 24 tests:

 Product Name

 Size (test/kit)


 Cat. No.

 JAK2 Mutation Detection Kit (Bulk)




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