BRAF V600 Mutations Detection Kit
Real-time PCR Assays
- Bulk formats are available.
- Bulk formats are available.
- Compatible with tissue.
- Compatible with tissue.
- Top sensitivity of 1% mutant allele.
- Top sensitivity of 1% mutant allele.
- One-step procedure, results in 90 mins.
- One-step procedure, results in 90 mins.
- Internal control included.
- Internal control included.
Product Description

BRAF V600 Mutations Detection Kit

The AmoyDx® BRAF V600 Mutations Detection Kit is a sensitive and selective assay for the detection of the V600 (1799T>A) mutation in the BRAF oncogene.

BRAF is a serine/threonine kinase that functions within the Ras-Raf-MEK-MAPK pathway. This pathway normally regulates cell proliferation and survival under the control of growth factors and hormones. Mutations in the BRAF gene have been associated with the development of cancer. The most common alteration in the BRAF gene is a mutation called V600E, which alters the valine at position 600 in the protein to a glutamic acid. The V600E mutation causes the BRAF protein to be permanently activated even in the absence of growth factors.

Aberrant BRAF signaling due to the V600 mutations can result in excessive cell proliferation and an adverse resistance to apoptosis. BRAF mutations occur in ~50% of melanoma tumors, ~40% of papillary thyroid tumors, ~30% of ovarian tumors, ~10% of colorectal tumors and ~10% of prostate tumors. Drugs that block oncogenic BRAF signaling have had impressive results in clinical trials and the search for new BRAF-targeted drugs is an active area of research and development.

The AmoyDx® BRAF V600 Mutations Detection Kit is a highly selective and sensitive test for detecting the V600 mutations in the BRAF oncogene. AmoyDx's patented technology allows detection of 1% mutant DNA in a background of 99% normal DNA, while ensuring that false negatives are minimized. The procedure is easily adapted for use in high-throughput sample processing.

Technological Principles:

The BRAF V600 mutations detection kit employs a real-time PCR format combined with patented technology - “mutation specific amplification technology” - to detect mutations in DNA samples. The method is much more specific and sensitive than alternative technologies such as DNA sequencing.

Testing Procedure
Compatible Sample Types:FFPE Samples
Compatible Real-time PCR Instrument
ABI 7500
ABI 7900HT
ABI StepOnePlus
Bio-Rad CFX96
LightCycler 480 II
Stratagene Mx3000P™
Other Info.

To order, please contact

 Product Name  Size (test/kit)  Storage Cat. No.
 BRAF V600 Mutations Detection Kit (Bulk)  24T  -20℃ 8.01.20302X024E

1. Li, Zhenxiang, et al. "Prevalence and clinical significance of BRAF V600E in Chinese patients with lung adenocarcinoma." Thoracic cancer 6.3 (2015): 269-274.
2. Ye, Ju-Xiang, et al. "KRAS and BRAF gene mutations and DNA mismatch repair status in Chinese colorectal carcinoma patients." World journal of gastroenterology: WJG 21.5 (2015): 1595.
3. Tuononen, Katja, et al. "Comparison of targeted next‐generation sequencing (NGS) and real‐time PCR in the detection of EGFR, KRAS, and BRAF mutations on formalin‐fixed, paraffin‐embedded tumor material of non‐small cell lung carcinoma—superiority of NGS." Genes, Chromosomes and Cancer 52.5 (2013): 503-511.
4. Kakavand, Hojabr, et al. "BRAFV600E and NRASQ61L/Q61R mutation analysis in metastatic melanoma using immunohistochemistry: a study of 754 cases highlighting potential pitfalls and guidelines for interpretation and reporting." Histopathology 69.4 (2016): 680-686.

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