KRAS Mutation Detection Kit
PCR
Real-time PCR Assays
19
Mutations
1%~5%
1%~5%
Sensitivity
Features
Selectable
- Pre-loaded and bulk formats are available.
1
- Pre-loaded and bulk formats are available.
Universal
- Compatible with FFPE tissue samples.
2
- Compatible with FFPE tissue samples.
Precise
- Top sensitivity of 1% mutant allele.
3
- Top sensitivity of 1% mutant allele.
Easy
- One-step procedure, results in 90 mins.
4
- One-step procedure, results in 90 mins.
Reliable
- Internal and external controls included.
5
- Internal and external controls included.
Product Description

KRAS Mutation Detection Kit
Detection of 19 mutations in KRAS codons 12, 13, 59, 61, 117 and 146
KRAS protein is a small GTPase and one of the key molecules in the downstream signaling pathway of epidermal growth factor receptor (EGFR). KRAS protein transduces signals from membrane-bound receptors via multiple downstream effector pathways and thereby affects fundamental cellular processes, including proliferation, apoptosis, and differentiation.
KRAS mutations are particularly common in colon cancer, lung cancer, and pancreatic cancer. In total, activating mutations in the KRAS genes occur in 15~30% of lung cancer and 20~50% of colorectal cancer, mainly in exons 2, 3 or 4.
The KRAS gene mutation status of colorectal cancer patients is relevant to drug resistance of targeted medicines such as tyrosine kinase inhibitors. Patients with wild-type KRAS gene are much more likely to benefit from the Erbitux (Cetuximab) or Vectibix (Panitumumab), compared to patients with a mutant KRAS gene. The European Drug Administration Organization and US FDA recommend the employment of a KRAS gene mutation test prior to the usage of targeted medicine Erbitux and and Vectibix in the treatment of colorectal cancer.
Technological Principles
The kit uses novel, patented primers and probes to detect mutations in a real-time PCR assay. The mutant DNA is amplified accurately by the specific primers, and detected by the novel probes. Highly specific primers and probes, and a highly validated procedure based on Taq DNA polymerase contribute to outstanding assay sensitivity and selectivity.
 

Testing Procedure
Compatible Sample Types:FFPE Samples
Compatible Real-time PCR Instrument
ABI7500
cobas z480
Bio-Rad CFX96
LightCycler480 II
Rotor-Gene 6000
Rotor-Gene Q
SLAN-96S
Stratagene Mx3000P™
Other Info.

To order, please contact sales@amoydx.com.

AmoyDx KRAS Mutation Detection Kits are available in the following size, minimum order of 6 tests:

 Product Name

Type

 Size (test/kit)

Strorage

 Cat. No.

KRAS Mutation Detection Kit

Pre-loaded

6T

-20±5 ℃

8.01.20102W006A
8.01.20102W006B
8.01.20102W006D

KRAS Mutation Detection Kit

Bulk

24T

-20±5 ℃

8.01.20102X024H


Publications
1. Ye, Ju-Xiang, et al. "KRAS and BRAF gene mutations and DNA mismatch repair status in Chinese colorectal carcinoma patients." World journal of gastroenterology: WJG 21.5 (2015): 1595.
2. Hagan, Suzanne, Maria CM Orr, and Brendan Doyle. "Targeted therapies in colorectal cancer—an integrative view by PPPM." EPMA Journal 4.1 (2013): 3.
3. Trachu, N., et al. "Molecular alterations and clinical prognostic factors for cholangiocarcinoma in Thai population." OncoTargets and therapy 10 (2017): 4955.

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